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. 2016 Oct 11;11:5257–5276. doi: 10.2147/IJN.S112030

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© 2016 Guo et al. This work is published and licensed by Dove Medical Press Limited

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The declined cell viability of HUVECs induced by SiNPs, and restored by NAC.

Notes: (A) The cytotoxicity of NAC on HUVECs assessed by MTT assay. Cells were treated with NAC for 24 hours at various concentrations, which were 0.125, 0.25, 0.5, 1, 2, 4, 5, 8, and 10 mM, respectively. Control received culture medium only. Finally, 5 mM was chosen as the dose of NAC for the following study. (B) Cell viability of HUVECs measured by MTT assay. Cells were pretreated with 5 mM NAC for 2 hours followed by exposure of SiNPs (12.5, 25, 50, and 100 μg/mL, respectively) for either 24 or 48 hours. All values are mean ± SD of three identical experiments made in triplicate. *P<0.05 vs control and ^#P<0.05 for SiNPs vs SiNPs plus NAC.

Abbreviations: HUVECs, human umbilical vein endothelial cells; SiNPs, silica nanoparticles; NAC, N-acetylcysteine; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; SD, standard deviation.