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. 2016 Oct 11;11:5257–5276. doi: 10.2147/IJN.S112030

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© 2016 Guo et al. This work is published and licensed by Dove Medical Press Limited

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Apoptosis promoted by SiNPs, and the prevention by NAC.

Notes: (A) AO/EB staining and flow cytometry using (B) Annexin V-FITC double-stain were used to detect the apoptosis of HUVECs after treatment with 50 μg/mL SiNPs for 24 hours plus NAC pretreatment (5 mM, 2 hours). Scale bar =50 μm, with the magnification of ×200. Data are expressed as mean ± SD from three independent experiments. *P<0.05 vs control; ^#P<0.05 for SiNPs vs SiNPs + NAC.

Abbreviations: SiNPs, silica nanoparticles; NAC, N-acetylcysteine; AO, acridine orange; EB, ethidium bromide; HUVECs, human umbilical vein endothelial cells; SD, standard deviation.