Table 1.
Biotinylated-peptides and biotinylated-Cys sites in sGC identified by LC/MS/MS.
| Protein Description |
Gene ID | Swiss-Prot Protein Accession |
Sequence | Modification | Therotical Mass |
Actual Mass |
Observed m/z |
Charges | ΔM (ppm) |
Best Mascot Score |
|---|---|---|---|---|---|---|---|---|---|---|
| sGC alpha | Gucy1a3 | P19686 | STKPSLSPGKPQSSLVIPTSLFCK | AC282 | 2929.5479 | 2929.5427 | 977.52 | 3+ | −1.7 | 59 |
| FDQQCGELDVYK | AC516 | 1871.8256 | 1871.8293 | 936.92 | 2+ | 1.5 | 52 | |||
| YCLFGNNVTLANK | AC594 | 1883.9096 | 1883.9117 | 942.96 | 2+ | 2.4 | 68 | |||
| FESCSVPR | AC609 | 1351.6087 | 1351.608 | 676.81 | 2+ | −1.7 | 38 | |||
| DCPGFVFTPR | AC628 | 1565.7193 | 1565.7209 | 783.87 | 2+ | −0.5 | 58 | |||
| sGC beta | Gucy1b3 | P20595 | SEECDHTQFLIEEK | BC174 | 2134.9373 | 2134.94 | 1068.48 | 2+ | 1.3 | 53 |
| ISPYTFCK | BC214 | 1385.6546 | 1385.6555 | 693.84 | 2+ | 0.7 | 29 | |||
| DLVVTQCGNAIYR | BC232 | 1878.9154 | 1878.9152 | 940.46 | 2+ | −0.1 | 42 | |||
| YCLFGNTVNLTSR | BC541 | 1914.9154 | 1914.9172 | 958.47 | 2+ | 0.9 | 46 | |||
| CLMSPENSDPQFHLEHR | BC571 | 2467.0905 | 2467.092 | 823.37 | 3+ | −0.3 | 36 |
*
Biotinylated-Vys609 was identified in both endogenous and CSNO treated sGC