Fig 2. IgG host species specificity of IgdE family proteases.
0.5 mg/ml human, porcine, equine, bovine and murine IgG were incubated for 16h at 37°C with (A) 20 μg/ml purified rIgdEagalactiae, (B) 20 μg/ml purified rIgdEporcinus, (C) 5% soluble fraction of E. coli cells expressing rIgdEpseudoporcinus, (D) 5% soluble fraction of E. coli cells expressing rIgdEequi. PBS (A and B) or 5% soluble fraction of E. coli cells without recombinant construct (C and D) were used as negative controls (-). Reactions were analyzed by Coomassie blue SDS-PAGE under reducing conditions. Images of different SDS-PAGE run in parallel have been assembled into one figure. The diagnostic 32 kDa IgG cleavage product (*) appeared when rIgdEagalactiae was incubated with human IgG, rIgdEporcinus with porcine IgG, rIgdEpseudoporcinus with human IgG and porcine IgG, and rIgdEequi with equine IgG.