Fig 3. Effect of nocodazole on RABV-induced SGs.

U373-MG cells were infected and treated with nocodazole (NCZ) (2 μM) or mock treated (DMSO) for 1 h before virus inoculation and during infection. At 20 h p.i as indicated, cells were co-stained with goat anti-TIA-1, mouse anti-α tubulin and rabbit anti-P antibodies, followed by incubation with a mixture of Alexa-488 donkey anti-goat IgG, Alexa-568 donkey anti-mouse IgG and Alexa-647 donkey anti-rabbit IgG. The scale bars correspond to 15 μm.