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. 2016 Oct 17;12(10):e1006383. doi: 10.1371/journal.pgen.1006383

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© 2016 Sethi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — (A) Maximum Z projection spinning disk confocal images of germinated cells of the indicated genotype from diploid sbg1Δ/sbg1⁺ mCherry-Atb2p Rlc1p-3GFP (MBY9156). Green, Rlc1p-GFP. Red, mCherry-Atb2p. (B) Maximum Z projection spinning disk confocal montage of the indicated strain (sbg1-3 GFP-Bgs1p Rlc1p-tdTomato Pcp1p-mCherry—MBY9390) after 6 hr at 36°C. Green, GFP-Bgs1p. Red, Rlc1p-tdTomato Pcp1p-mCherry. Calcofluor White (CW) images were acquired as single medial plane images and are inverted for fluorescence. 0min indicates time of spindle body duplication. Defects observed in 29.1%±7.9% cells (n = 3, at least 45 cells). (C) Maximum Z projection spinning disk confocal montage of the indicated strain (hyg^r-GFP-Sbg1-3p GFP-Bgs1p Rlc1p-tdTomato Pcp1p-mCherry—MBY9389) after 6 hr at 36°C. Green, hyg^r-GFP-Sbg1-3p. Red, Rlc1p-tdTomato Pcp1p-mCherry. Calcofluor White (CW) images were acquired as single medial plane images and are inverted for fluorescence. 0min indicates time of spindle body duplication. Defects observed in 37.4%±6.7% cells (n = 2, at least 20 cells). Scale bar 5μm.