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. 2016 Oct 17;11(10):e0163471. doi: 10.1371/journal.pone.0163471

Table 2. Response using EIC peak areas of each external standards over increasing concentrations.

aCN (S1+S2) bCN (A1+A2+B+I) kCN (A+B) aLA (B, B+G) bLG (A+B+D) BSA
Concentration Mean SD Mean SD Mean SD Mean SD Mean SD Mean SD
0.25 0.261 0.020 0.812 0.099 0.590 0.016 2.823 0.082 0.719 0.010 0.371 0.031
0.50 0.651 0.015 1.808 0.201 1.259 0.023 4.248 0.131 1.759 0.041 0.683 0.082
0.75 1.037 0.020 3.523 0.324 1.977 0.014 6.598 0.157 2.832 0.058 1.061 0.102
1.00 1.484 0.035 5.103 0.444 2.920 0.007 10.825 0.236 3.910 0.116 1.213 0.204
2.50 2.893 0.086 13.423 0.603 6.693 0.101 21.581 0.340 8.306 0.246 2.268 0.306
5.00 5.483 0.191 21.769 1.121 11.697 0.317 36.286 0.540 17.363 0.479 3.309 0.408
7.50 8.028 0.311 28.721 1.521 15.247 0.585 50.038 0.626 23.026 0.693 4.337 0.510
10.00 11.053 0.396 33.320 1.896 18.672 0.891 58.970 0.789 27.211 0.913 5.001 0.612
SLOPE 1.074 3.447 1.882 5.916 2.814 0.469
SE 0.163 2.360 0.943 2.963 1.376 0.329
INTERCEPT 0.170 1.709 0.913 3.583 0.969 0.668
R2 0.999 0.971 0.984 0.984 0.985 0.970
LOD (mg/mL) 0.457 2.054 1.504 1.502 1.468 2.102
LOQ (mg/mL) 1.522 6.846 5.012 5.008 4.892 7.006
Working range 0.5–10 mg/mL 2.0–10 mg/mL 1.5–10 mg/mL 1.5–10 mg/mL 1.5–10 mg/mL 2.1–10 mg/mL

External standards were run in duplicates. Based on the averaged results, the slope, standard error (SE), intercept, Pearson correlation coefficient (R2) values, limits of detection (LOD) and quantitation (LOQ), and working range were computed. LOD for each standard was obtained using the following formula: 3*(standard error/slope). LOQ for each standard was obtained using the following formula: 10*(standard error/slope). The working range was the interval between the LOQ and the upper concentration of the analyte in the samples tested in this study (10 mg/mL) for which linearity was demonstrated.