Table 2. Response using EIC peak areas of each external standards over increasing concentrations.
aCN (S1+S2) | bCN (A1+A2+B+I) | kCN (A+B) | aLA (B, B+G) | bLG (A+B+D) | BSA | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Concentration | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD |
0.25 | 0.261 | 0.020 | 0.812 | 0.099 | 0.590 | 0.016 | 2.823 | 0.082 | 0.719 | 0.010 | 0.371 | 0.031 |
0.50 | 0.651 | 0.015 | 1.808 | 0.201 | 1.259 | 0.023 | 4.248 | 0.131 | 1.759 | 0.041 | 0.683 | 0.082 |
0.75 | 1.037 | 0.020 | 3.523 | 0.324 | 1.977 | 0.014 | 6.598 | 0.157 | 2.832 | 0.058 | 1.061 | 0.102 |
1.00 | 1.484 | 0.035 | 5.103 | 0.444 | 2.920 | 0.007 | 10.825 | 0.236 | 3.910 | 0.116 | 1.213 | 0.204 |
2.50 | 2.893 | 0.086 | 13.423 | 0.603 | 6.693 | 0.101 | 21.581 | 0.340 | 8.306 | 0.246 | 2.268 | 0.306 |
5.00 | 5.483 | 0.191 | 21.769 | 1.121 | 11.697 | 0.317 | 36.286 | 0.540 | 17.363 | 0.479 | 3.309 | 0.408 |
7.50 | 8.028 | 0.311 | 28.721 | 1.521 | 15.247 | 0.585 | 50.038 | 0.626 | 23.026 | 0.693 | 4.337 | 0.510 |
10.00 | 11.053 | 0.396 | 33.320 | 1.896 | 18.672 | 0.891 | 58.970 | 0.789 | 27.211 | 0.913 | 5.001 | 0.612 |
SLOPE | 1.074 | 3.447 | 1.882 | 5.916 | 2.814 | 0.469 | ||||||
SE | 0.163 | 2.360 | 0.943 | 2.963 | 1.376 | 0.329 | ||||||
INTERCEPT | 0.170 | 1.709 | 0.913 | 3.583 | 0.969 | 0.668 | ||||||
R2 | 0.999 | 0.971 | 0.984 | 0.984 | 0.985 | 0.970 | ||||||
LOD (mg/mL) | 0.457 | 2.054 | 1.504 | 1.502 | 1.468 | 2.102 | ||||||
LOQ (mg/mL) | 1.522 | 6.846 | 5.012 | 5.008 | 4.892 | 7.006 | ||||||
Working range | 0.5–10 mg/mL | 2.0–10 mg/mL | 1.5–10 mg/mL | 1.5–10 mg/mL | 1.5–10 mg/mL | 2.1–10 mg/mL |
External standards were run in duplicates. Based on the averaged results, the slope, standard error (SE), intercept, Pearson correlation coefficient (R2) values, limits of detection (LOD) and quantitation (LOQ), and working range were computed. LOD for each standard was obtained using the following formula: 3*(standard error/slope). LOQ for each standard was obtained using the following formula: 10*(standard error/slope). The working range was the interval between the LOQ and the upper concentration of the analyte in the samples tested in this study (10 mg/mL) for which linearity was demonstrated.