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. 2016 Oct 17;11(10):e0164725. doi: 10.1371/journal.pone.0164725

Table 1. Sequence information of the templates and primers for qRT-PCR.

miRNA Gene description miRNA mature sequence (5'-3') Forward primer (5'-3') Amplication efficiency (%) R2 Reference
Cla-miR81 microRNA ATGTCTATCTGGGTCTATCGCAGT ATGTCTATCTGGGTCTATCGCAG 97.916 0.985 Selected from small RNA-seq
Cla-miR82 microRNA ATTGTTGTTACATAAAGGACGAGT ATTGTTGTTACATAAAGGACGAG 83.609 0.985 Selected from small RNA-seq
Cla-miR167f microRNA TGAAGCTGCCAGCATGATCTG TGAAGCTGCCAGCATGATCTG 108.05 0.996 [18,21]
Cmo-miR167f microRNA TGAAGCTGCCAGCATGATCTG TGAAGCTGCCAGCATGATCTG 108.127 0.998 [18,21]
Lsi-miR167f microRNA TGAAGCTGCCAGCATGATCTG TGAAGCTGCCAGCATGATCTG 109.097 0.998 [18,21]
Cla-miR166b microRNA TCGGACCAGGCTTCATTCCCC TCGGACCAGGCTTCATTCCCC 104.928 1 Selected from small RNA-seq
Cmo-miR166b microRNA TCGGACCAGGCTTCATTCCCC TCGGACCAGGCTTCATTCCCC 111.222 1 Selected from small RNA-seq
Lsi-miR166b microRNA TCGGACCAGGCTTCATTCCCC TCGGACCAGGCTTCATTCCCC 106.805 0.998 Selected from small RNA-seq
Cla-miR169n-5p microRNA TAGCCAAAAATGACTTGCCTGC GGTAGCCAAAAATGACTTGCCTGC 102.642 0.993 [24]
Cla-miR170 microRNA TGATTGAGCCGCGCCAATATC CTGATTGAGCCGCGCCAATATC 104.323 0.998 Selected from small RNA-seq
Cla-miR167c microRNA TGAAGCTGCCAGCATGATCTT TGAAGCTGCCAGCATGATCTT 105.961 0.996 [18,21]
Cmo-miR167c microRNA TGAAGCTGCCAGCATGATCTT TGAAGCTGCCAGCATGATCTT 103.039 0.998 [18,21]
Lsi-miR167c microRNA TGAAGCTGCCAGCATGATCTT TGAAGCTGCCAGCATGATCTT 101.089 0.997 [18,21]
Cmo-miR3511-3p microRNA AGTTACTAATTAATGATCTGGC AGTTACTAATTAATGATCTGGC 96.331 0.988 Selected from small RNA-seq
Cmo-miR167b microRNA TGAAGCTGCCAGCATGATCTA TGAAGCTGCCAGCATGATCT 107.682 0.997 [18,21]
Cmo-miR160a microRNA TGCCTGGCTCCCTGTATGCC TGCCTGGCTCCCTGTATGCC 109.31 1 [25]
Cmo-miR319b microRNA TGCCTGGCTCCCTGTATGCC TCGTTGGACTGAAGGGAGC 108.429 0.996 Selected from small RNA-seq
Lsi-miR398b microRNA TGTGTTCTCAGGTCGCCCCTA TGTGTTCTCAGGTCGCCCCT 107.615 0.998 Selected from small RNA-seq
Lsi-miR166u microRNA TCTCGGACCAGGCTTCATTCT CTCGGACCAGGCTTCATTCTA 109.964 1 Selected from small RNA-seq
Cmo-miR397a microRNA TCATTGAGTGCAGCGTTGATG TCATTGAGTGCAGCGTTGATG 114.628 0.991 Selected from small RNA-seq
Cla-miR164a microRNA TGGAGAAGCAGGGCACGT TGGAGAAGCAGGGCACGT 93.059 1 Selected from small RNA-seq
Lsi-miR5148a microRNA GGAGGGGTGCTTGCCTAAGGTCTG GGAGGGGTGCTTGCCTAAGGTCTG 98.74 0.998 Selected from small RNA-seq
miR85 microRNA AGGACTTTGAAAAGAAAGA GGAGGACTTTGAAAAGAAAG 105.477 0.987 Selected from small RNA-seq
universal reverse primer provided with the kit
ncRNA Gene description Forward primer (5'-3') Reverse primer (5'-3') Amplication efficiency (%) R2 Reference
Cla-U6 small nuclear RNA GGGGACATCCGATAAAATT TGTGCGTGTCATCCTTGC 101.47 0.998 [28]
Cmo-U6 small nuclear RNA GGGGACATCCGATAAAATT TGTGCGTGTCATCCTTGC 109.956 0.998 [28]
Lsi-U6 small nuclear RNA GGGGACATCCGATAAAATT TGTGCGTGTCATCCTTGC 109.798 0.999 [28]
Cla-18S ribosome RNA AGCCTGAGAAACGGCTACCACATC ACCAGACTCGAAGAGCCCGGTAT 96.564 0.983 [27]
Cmo-18S ribosome RNA AGCCTGAGAAACGGCTACCACATC ACCAGACTCGAAGAGCCCGGTAT 107.15 0.994 [27]
Lsi-18S ribosome RNA AGCCTGAGAAACGGCTACCACATC ACCAGACTCGAAGAGCCCGGTAT 97.636 0.993 [27]
mRNA Gene description Forward primer (5'-3') Reverse primer (5'-3') Amplication efficiency (%) R2 Reference
ClYLS8 Yellow-leaf-specific proein8 AGAACGGCTTGTGGTCATTC GAGGCCAACACTTCATCCAT 105.159 0.995 [20]
CmYLS8 Yellow-leaf-specific proein8 AGAACGGCTTGTGGTCATTC GAGGCCAACACTTCATCCAT 99.648 0.994 [20]
CmPP2A-1 Protein phosphatase 2A regulatory subunit A AAGAGCCCACCAGCTTGTAA TGTTCTCCCCAATCTCAAGG 96.114 0.993 [20]
LsPP2A-2 Protein phosphatase 2A regulatory subunit A TGGTAGCATCCTTTCCCAATACA CATGCCCGTTCAGCTTTAGC 106.171 0.999 [26]