miRNA |
Gene description |
miRNA mature sequence (5'-3') |
Forward primer (5'-3') |
Amplication efficiency (%) |
R2
|
Reference |
Cla-miR81 |
microRNA |
ATGTCTATCTGGGTCTATCGCAGT |
ATGTCTATCTGGGTCTATCGCAG |
97.916 |
0.985 |
Selected from small RNA-seq |
Cla-miR82 |
microRNA |
ATTGTTGTTACATAAAGGACGAGT |
ATTGTTGTTACATAAAGGACGAG |
83.609 |
0.985 |
Selected from small RNA-seq |
Cla-miR167f |
microRNA |
TGAAGCTGCCAGCATGATCTG |
TGAAGCTGCCAGCATGATCTG |
108.05 |
0.996 |
[18,21] |
Cmo-miR167f |
microRNA |
TGAAGCTGCCAGCATGATCTG |
TGAAGCTGCCAGCATGATCTG |
108.127 |
0.998 |
[18,21] |
Lsi-miR167f |
microRNA |
TGAAGCTGCCAGCATGATCTG |
TGAAGCTGCCAGCATGATCTG |
109.097 |
0.998 |
[18,21] |
Cla-miR166b |
microRNA |
TCGGACCAGGCTTCATTCCCC |
TCGGACCAGGCTTCATTCCCC |
104.928 |
1 |
Selected from small RNA-seq |
Cmo-miR166b |
microRNA |
TCGGACCAGGCTTCATTCCCC |
TCGGACCAGGCTTCATTCCCC |
111.222 |
1 |
Selected from small RNA-seq |
Lsi-miR166b |
microRNA |
TCGGACCAGGCTTCATTCCCC |
TCGGACCAGGCTTCATTCCCC |
106.805 |
0.998 |
Selected from small RNA-seq |
Cla-miR169n-5p |
microRNA |
TAGCCAAAAATGACTTGCCTGC |
GGTAGCCAAAAATGACTTGCCTGC |
102.642 |
0.993 |
[24] |
Cla-miR170 |
microRNA |
TGATTGAGCCGCGCCAATATC |
CTGATTGAGCCGCGCCAATATC |
104.323 |
0.998 |
Selected from small RNA-seq |
Cla-miR167c |
microRNA |
TGAAGCTGCCAGCATGATCTT |
TGAAGCTGCCAGCATGATCTT |
105.961 |
0.996 |
[18,21] |
Cmo-miR167c |
microRNA |
TGAAGCTGCCAGCATGATCTT |
TGAAGCTGCCAGCATGATCTT |
103.039 |
0.998 |
[18,21] |
Lsi-miR167c |
microRNA |
TGAAGCTGCCAGCATGATCTT |
TGAAGCTGCCAGCATGATCTT |
101.089 |
0.997 |
[18,21] |
Cmo-miR3511-3p |
microRNA |
AGTTACTAATTAATGATCTGGC |
AGTTACTAATTAATGATCTGGC |
96.331 |
0.988 |
Selected from small RNA-seq |
Cmo-miR167b |
microRNA |
TGAAGCTGCCAGCATGATCTA |
TGAAGCTGCCAGCATGATCT |
107.682 |
0.997 |
[18,21] |
Cmo-miR160a |
microRNA |
TGCCTGGCTCCCTGTATGCC |
TGCCTGGCTCCCTGTATGCC |
109.31 |
1 |
[25] |
Cmo-miR319b |
microRNA |
TGCCTGGCTCCCTGTATGCC |
TCGTTGGACTGAAGGGAGC |
108.429 |
0.996 |
Selected from small RNA-seq |
Lsi-miR398b |
microRNA |
TGTGTTCTCAGGTCGCCCCTA |
TGTGTTCTCAGGTCGCCCCT |
107.615 |
0.998 |
Selected from small RNA-seq |
Lsi-miR166u |
microRNA |
TCTCGGACCAGGCTTCATTCT |
CTCGGACCAGGCTTCATTCTA |
109.964 |
1 |
Selected from small RNA-seq |
Cmo-miR397a |
microRNA |
TCATTGAGTGCAGCGTTGATG |
TCATTGAGTGCAGCGTTGATG |
114.628 |
0.991 |
Selected from small RNA-seq |
Cla-miR164a |
microRNA |
TGGAGAAGCAGGGCACGT |
TGGAGAAGCAGGGCACGT |
93.059 |
1 |
Selected from small RNA-seq |
Lsi-miR5148a |
microRNA |
GGAGGGGTGCTTGCCTAAGGTCTG |
GGAGGGGTGCTTGCCTAAGGTCTG |
98.74 |
0.998 |
Selected from small RNA-seq |
miR85 |
microRNA |
AGGACTTTGAAAAGAAAGA |
GGAGGACTTTGAAAAGAAAG |
105.477 |
0.987 |
Selected from small RNA-seq |
universal reverse primer |
provided with the kit |
|
ncRNA |
Gene description |
Forward primer (5'-3') |
Reverse primer (5'-3') |
Amplication efficiency (%) |
R2
|
Reference |
Cla-U6 |
small nuclear RNA |
GGGGACATCCGATAAAATT |
TGTGCGTGTCATCCTTGC |
101.47 |
0.998 |
[28] |
Cmo-U6 |
small nuclear RNA |
GGGGACATCCGATAAAATT |
TGTGCGTGTCATCCTTGC |
109.956 |
0.998 |
[28] |
Lsi-U6 |
small nuclear RNA |
GGGGACATCCGATAAAATT |
TGTGCGTGTCATCCTTGC |
109.798 |
0.999 |
[28] |
Cla-18S |
ribosome RNA |
AGCCTGAGAAACGGCTACCACATC |
ACCAGACTCGAAGAGCCCGGTAT |
96.564 |
0.983 |
[27] |
Cmo-18S |
ribosome RNA |
AGCCTGAGAAACGGCTACCACATC |
ACCAGACTCGAAGAGCCCGGTAT |
107.15 |
0.994 |
[27] |
Lsi-18S |
ribosome RNA |
AGCCTGAGAAACGGCTACCACATC |
ACCAGACTCGAAGAGCCCGGTAT |
97.636 |
0.993 |
[27] |
mRNA |
Gene description |
Forward primer (5'-3') |
Reverse primer (5'-3') |
Amplication efficiency (%) |
R2
|
Reference |
ClYLS8 |
Yellow-leaf-specific proein8 |
AGAACGGCTTGTGGTCATTC |
GAGGCCAACACTTCATCCAT |
105.159 |
0.995 |
[20] |
CmYLS8 |
Yellow-leaf-specific proein8 |
AGAACGGCTTGTGGTCATTC |
GAGGCCAACACTTCATCCAT |
99.648 |
0.994 |
[20] |
CmPP2A-1 |
Protein phosphatase 2A regulatory subunit A |
AAGAGCCCACCAGCTTGTAA |
TGTTCTCCCCAATCTCAAGG |
96.114 |
0.993 |
[20] |
LsPP2A-2 |
Protein phosphatase 2A regulatory subunit A |
TGGTAGCATCCTTTCCCAATACA |
CATGCCCGTTCAGCTTTAGC |
106.171 |
0.999 |
[26] |