Figure 4.

Augmentation of lapatinib-induced autophagy by everolimus enhances the cytocidal effect of lapatinib in NPC cells.

Notes: (A) CNE-2 and HONE-1 cells were treated with lapatinib (0–10 μM) or DMSO control for 48 hours in the presence or absence of 5 μM of Everolimus. Cell viability was then assessed by CCK-8 assay. Results shown were mean ± standard deviation. *P<0.05; **P<0.01. (B) IC₅₀ values are presented as the mean ± standard deviation. (C) CNE-2 and HONE-1 cells were treated with lapatinib (0–5 μM) or DMSO control for 48 hours in the presence or absence of 5 μM of Everolimus, followed by western blotting to examine phospho-mTOR, phospho-PRS6, phospho-eEF2, and LC3 A/B. GAPDH was used as a loading control.