Skip to main content
. 2016 Oct 11;9:6151–6160. doi: 10.2147/OTT.S108890

Figure 4.

Figure 4

TPD52 downregulation reduced cell proliferation and induced apoptosis G1 phase arrest.

Notes: (A) TPD52 siRNA reduced the expression of TPD52. Cells were treated with siRNA or NC and incubated for 72 hours. TPD52 protein expression was determined by Western blot. (B) TPD52 downregulation reduced cell proliferation. Cells were treated with siRNA or NC and incubated for 48 hours. The cell viability at different time points was determined by CCK-8 assay. **P<0.01, compared with blank control and NC. (C) TPD52 downregulation induced cell apoptosis. Cells were treated with siRNA or NC and incubated for 72 hours. Apoptosis was determined by Annexin V/PI assay (**P<0.01). (D) TPD52 downregulation induced G1 phase arrest. Cells were treated with siRNA or NC and incubated for 72 hours. Cells were strained by PI and cell cycle was determined by flow cytometry (*P<0.05).

Abbreviations: CCK-8, Cell Counting Kit-8; NC, negative control; OD, optical density; PI, propidium iodide.