Cytochrome P 3A4 (CYP3A4) localization in human temporal lobe epileptic (TLE) brain. Representative images showing CYP3A4 localized in the cerebrovasculature (A) and neurons (B) of a human drug-resistant epileptic brain evaluated by immunohistochemistry. The autoptic brain tissues obtained from cardiomyopathic subjects (non-neurological case) showed lower levels of CYP3A4 compared with drug-resistant epileptic brains (C,D). Colocalization was evaluated with a neuronal marker staining the neuronal nuclei [NEUN (B)] and glial marker [Glial fibrillary acidic protein, GFAP (A,C)]. DAPI (4’,6-diamidino-2-phenylindole (D) was used as nuclear counterstain. Data from [7,8].