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. Author manuscript; available in PMC: 2017 Oct 28.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Sep 28;479(4):787–792. doi: 10.1016/j.bbrc.2016.09.131

Fig. 3. The SH3 domain is indispensable for IRTKS-mediated p38 phosphorylation.

Fig. 3

(A) Hela cells expressing GFP, IRTKS-GFP, and IRTKSΔSH3-GFP were treated with 10% FBS for 5 min and subjected to Western blot analysis for the level of phosphorylated p38. As a negative control, IRTKS-GFP cells were pre-treated with 10 μM SB203580 1 hr prior to adding serum. (B) Phosphorylation of Erk1/2 in cells expressing IRTKS-GFP or IRTKSΔSH3-GFP was evaluated after treated with 10% FBS for the indicated times. (C) Cells expressing IRTKS mutants were analyzed for the chemotactic response to serum in the presence and absence of 10 μM SB203580. ***, P<0.001, n=3.