TABLE 1.

Sequences of specific primers used for real-time qRT-PCR amplification

Name	Primer sequence, 5′–3′		Amplicon size (bp)
	Forward	Reverse
GAPDH	CCGTAGACAAAATGGTGAAGG	TCGTTGATGGCAACAATCTC	109
B2M	GGAGAATGGGAAGCCGAACA	TCTCGATCCCAGTAGACGGT	249
RPS29	ATGGGTCACCAGCAGCTCTA	GTATTTGCGGATCAGACCGT	102
COX I	CACTAATAATCGGAGCCCCA	TTCATCCTGTTCCTGCTCCT	129
COX IV	TGGGAGTGTTGTGAAGAGTGA	GCAGTGAAGCCGATGAAGAAC	273
CS	CAAGATTGTGCCCAATATCCTC	TTCATCTCCGTCATGCCATA	111
MCIP1	CAGCGAAAGTGAGACCAGGG	ACGGGGGTGGCATCTTCTAC	309
TFAM	GCTGATGGGTATGGAGAAG	GAGCCGAATCATCCTTTGC	161
PGC-1α	TTCCACCAAGAGCAAGTAT	CGCTGTCCCATGAGGTATT	131
NRF1	TTACTCTGCTGTGGCTGATGG	CCTCTGATGCTTGCGTCGTCT	92
OPA1	TCAGCAAAGCTTACATGCAGA	TGCTTGGACTGGCTACATTTT	180
MFN1	TGCCCTCTTGAGAGATGACC	AGAGCCGCTCATTCACCTTA	182
MFN2	GGGGCCTACATCCAAGAGAG	CCTTGGACAGGTACCCTTTG	115
FIS1	GCCTGGTTCGAAGCAAATAC	CACGGCCAGGTAGAAGACAT	116
DRP1	CTGACGCTTGTGGATTTACC	CCCTTCCCATCAATACATCC	277
ND1	CCTATCACCCTTGCCATCAT	GAGGCTGTTGCTTGTGTGAC	194
PECAM 1	ATGGAAAGCCTGCCATCATG	TCCTTGTTGTTCAGCATCAC	235

COX I, cytochrome c oxidase subunit I; COX IV, cytochrome c oxidase subunit IV; DRP1, dynamin-related protein 1; FIS1, mitochondrial fission 1 protein; MCIP1, modulatory calcineurin interacting protein 1; MFN1, mitofusin 1; MFN2, mitofusin 2; ND1, mitochondrial encoded NADH dehydrogenase 1; NRF1, nuclear respiratory factor 1; OPA1, optic atrophy type 1; PECAM 1, platelet endothelial cell adhesion molecule 1; TFAM, mitochondrial transcription factor A.