Figure 3.

Requirement of Miro1 for Dendritic Maintenance

(A and B) Mitochondrial distribution in WT and Miro1^CKO CA1 neurons at 4 and 8 months of age (A) and normalized mitochondrial index (B) (n = images: at 4 months WT = 23, Miro1^CKO = 23; at 8 months WT = 22, Miro1^CKO = 20; t test).

(C and D) Dendritic segments from the stratum lacunosum-moleculare of AAV infected animals at 4 months (C). Quantification of the dendritic length not occupied by mitochondria (D) in WT and Miro1^CKO.

(E–J) Reconstructed traces (E), branchpoint Sholl analysis (F), dendritic length (G), and number of branchpoints (H) from WT and Miro1^CKO Golgi-stained CA1 neurons at 8 months of age. (I) Normalized BP⁹⁰ value for branching along the apical dendrite. (J) Subtracted (WT-Miro1^CKO) plot of the Sholl distribution (n = cells: WT = 15, Miro1^CKO = 14; ANOVA-NK).

(K) FluoroNissl staining of brain coronal sections showing the hippocampus, cortex, and lateral ventricles of 8-month-old WT and Miro1^CKO brains (scale bar, 500 μm). Right panels show the structure of the cerebral cortex in the somatosensory area (scale bar, 100 μm).

(L and M) Quantification of hippocampal (yellow arrows) (L) and cortical thickness (M) from brains at 8 months (n = animals: WT = 4, Miro1^CKO = 4, Miro2^KO = 4; ANOVA-NK).

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Error bars are SEM.