Figure 2.

MyT1 Promotes Neurogenesis in the Developing Telencephalon

(A) Analysis by western blot of MyT1 in P19 cells co-transfected with MyT1 expression vector, scramble shRNA (shControl), and/or MyT1 shRNA (shMyT1). α-tubulin was used as a loading control.

(B and C) In utero electroporation of control or MyT1-, control shRNA- (shControl), or MyT1 shRNA- (shMyT1) expressing vectors in E12.5 mouse ventral telencephalon. Immunofluorescence analysis on a coronal section of the telencephalon for GFP (green or gray) and TuJ1 (red) 2 days post-electroporation (E14.5) is shown. Cell nuclei are labeled with DAPI (blue). Histograms represent the quantification of VZ to SVZ transition based on the fraction of GFP⁺ cells that are retained in the VZ (GFP⁺VZ/GFP⁺) (B) and of neuronal differentiation based on the fraction of GFP⁺ cells that express TuJ1 ((TuJ1⁺GFP⁺)/GFP⁺) (C). VZ, ventricular zone; SVZ, subventricular zone. Scale bars, 20 and 50 μm (B and C).

Data are shown as mean ± SD; ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001, according to Student’s t test (B and C). See also Figure S2.