Figure 4.

MyT1 Counteracts Inhibition of Neuronal Differentiation by Notch Signaling

(A) Immunocytochemical analysis of Sox2 (red) and TuJ1 (green) upon infection with control or Ascl1-, Act Notch-, and/or MyT1-inducible lentiviruses 48 hr post-Dox. Nuclei were labeled with DAPI (blue). Histograms represent the percentage of Sox2⁺/DAPI or TuJ1⁺/DAPI cells in each condition. Scale bar, 40 μm.

(B and C) In utero electroporation of control or MyT1, Act Notch, or Act Notch + MyT1 expression vectors in E13.5 mouse dorsal telencephalon. Immunofluorescence analysis of coronal sections of GFP (green) and the neuronal marker Satb2 (red) 5 days after electroporation (E18.5) is shown. Cell nuclei are labeled with DAPI (blue). Scale bars, 500 μm (B) and 50 and 20 μm (C, left and right). (B’) Histogram represents the percentage of electroporated cells present in five bins (1 to 5 from ventricular to pial surface, respectively) of equal length spanning the cortical thickness. (C’) Histogram represents the percentage of (Satb2⁺GFP⁺)/GFP⁺ cells in each condition. Arrowheads indicate examples of Satb2⁺GFP⁺ cells. Arrows indicate examples of Satb2⁻GFP⁺ cells.

Data are shown as mean ± SD; n.s., p > 0.05; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001, according to one-way ANOVA with Bonferroni correction for multiple testing (A) and Student’s t test (B’–C’).