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. 2016 Oct 4;2016:9536192. doi: 10.1155/2016/9536192

Figure 6.

Figure 6

BMP4 induces the expression of Stra8 and c-Kit cooperatively with RA in cultured spermatogonia. (a-b) Real-time PCR analysis of Stra8 expression in cultured spermatogonia pretreated with BMP4 or Noggin and followed by RA stimulation for 8 hours (a) or 24 hours (b). (c-d) Real-time PCR analysis of c-Kit expression in cultured spermatogonia pretreated with BMP4 or Noggin and followed by RA stimulation for 8 hours (c) or 24 hours (d). (e) Real-time PCR analysis of BMP4 mRNA expression in cultured spermatogonia followed by RA alone stimulation for 8 hours and 12 hours. The columns were labelled with A, B, C, and D, which means values are significantly different (P < 0.05) among differently labelled groups; ∗ P < 0.05 or ∗∗ P < 0.01.