Table 2.

Fragments formed in vitro during incubation of 33-mer in mouse plasma, as determined by MALDI-TOF MS.

	LQLQPFPQPELPYPQPELPYPQPELPYPQPQPF	Sample
1	YPQPQPF	Filtrate
2	ELPYPQPEL	Filtrate
3	QPFPQPELPY	Filtrate
4	YPQPELPYPQPQPF	Filtrate
5	ELPYPQPELPYPQPEL	Filtrate
6	LQLQPFPQPELPYPQPE	4–6 kDa
7	ELPYPQPELPYPQPELPY	Filtrate
8	ELPYPQPELPYPQPELPYPQPQPF	14–18 kDa
9	FPQPELPYPQPELPYPQPELPYPQPQPF	10–12 kDa
10	PFPQPELPYPQPELPYPQPELPYPQPQPF	10–12 kDa
11	LQLQPFPQPELPYPQPELPYPQPELPYPQP	14–18 kDa
12	QPFPQPELPYPQPELPYPQPELPYPQPQPF	14–18 kDa
13	QLQPFPQPELPYPQPELPYPQPELPYPQPQP	14–17 kDa
14	LQLQPFPQPELPYPQPELPYPQPELPYPQPQ	14–17 kDa
15	LQLQPFPQPELPYPQPELPYPQPELPYPQPQP	4–6 kDa
16	LQLQPFPQPELPYPQPELPYPQPELPYPQPQPF	4–6 kDa, 6–9 kDa, 14–17 kDa

33-mer was incubated at 37°C in mouse plasma, and samples were filtered through 5 kDa MWCO centrifugal filters or run on SDS-PAGE gels before MALDI-TOF MS analysis. Approximate ranges of the gel slices, according to the molecular weight marker, are shown, and assignment of observed masses to 33-mer fragments was done as described in Table 1. The appearance of bands at 10–20 kDa on SDS-PAGE gels during incubation was confirmed before analysis of gel slices (not shown). The identified fragments at 10–18 kDa were not found in plasma where 33-mer was not added or when sampled at t = 0.