Skip to main content
. 2016 Oct 4;2016:3453059. doi: 10.1155/2016/3453059

Figure 4.

Figure 4

Inhibition of COX-2 activity attenuates H/R-induced cell injury and apoptosis in primary adult rat cardiomyocytes. (a) The release of LDH and (b) cell viability in primary adult rat cardiomyocytes with or without H/R treatment (45 min hypoxia followed by 2 h reoxygenation) in the presence or absence of NS398. The LDH leakage and cell viability were expressed against those in CTL group. (c) Representative original Western blots of cleaved caspase-3 and total caspase-3 in primary adult rat cardiomyocytes with or without H/R treatment in the presence or absence of NS398. Quantification of cleaved caspase-3 was normalized to total caspase-3. Data are shown as means ± SEM; ∗ P < 0.05 CTL versus H/R, # P < 0.05 H/R versus H/R + NS398 (one-way ANOVA followed by Tukey's test in (a), (b) and nonparametric Mann–Whitney U test in (c)); n = 5 per group.