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. 2016 Oct 4;2016:3453059. doi: 10.1155/2016/3453059

Figure 7.

Figure 7

Inhibition of COX-2 activity protects against H/R-induced cell apoptosis via Akt-dependent enhancement of iNOS expression. (a) Representative original Western blots of total Akt, phosphorylated Akt (T450), iNOS, cleaved caspase-3, and total caspase-3 in H9C2 cardiomyocytes with or without H/R stimulation in the presence or absence of NS398 or LY294002 (Akt-specific inhibitor, 10 μM, 1 h). Phosphorylated Akt, iNOS, and cleaved caspase-3 were normalized to total Akt, GAPDH, and total caspase-3, respectively. (b) The release of LDH, (c) cell viability, and (d) NO content were determined in H9C2 cardiomyocytes with or without H/R stimulation in the presence or absence of NS398 or LY294002 and were expressed against those in CTL group. Data are shown as means ± SEM; ∗ P < 0.05 CTL versus H/R or H/R + NS398 or H/R + NS398 + LY294002, # P < 0.05 H/R versus H/R + NS398, and Δ P < 0.05 H/R + NS398 versus H/R + NS398 + LY294002 (nonparametric Mann–Whitney U test in (a) and one-way ANOVA followed by Tukey's test in (b), (c), and (d)); n = 5 per group.