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In vitro ubiquitin conjugation assay of BdUev1s using purified proteins. After ubiquitination reactions as described, samples were subjected to SDS-PAGE and Western blotting using an anti-Ub antibody to monitor poly-Ub chain formation. rhUb, recombinant human ubiquitin. Asterisks indicate the non-specific bands in lanes 4, 7, and 10.
