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. 2016 Oct 18;7:1547. doi: 10.3389/fpls.2016.01547

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Copyright © 2016 Xiao, Sun, Liu, Li, He, Chen and Su.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pairwise variation (V) calculated through GeNorm to determine the optimal number of reference genes. The average pairwise variation (V_n/V_n+1) between normalization factors NF_n and NF_n+1was analyzed to determine the optimal number of reference genes required for qRT-PCR data normalization in different samples. (A) Different tissues, (B) flower samples of different developmental stages, and (C) all samples.