Figure 1. MASC-Seq overview.

A FACS machine sorts single cells onto a barcoded microarray, printed with six replicates on an activated glass slide. The throughput of the method and microarray design as a 33 × 35 ID matrix is illustrated. An alternative is to pipette and smear cells which then distribute randomly onto the array. Positions of the cells and IDs are noted in a high-resolution image and cDNA is only transcribed when an individual cell lands on top of the barcoded oligo-dTVN primer (ID).