Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 18;6:35599. doi: 10.1038/srep35599

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Identification of AIs in TGC proteins by pI4–7 (a–c) and pI6–9 (d–f) 2D gel electrophoresis. Numbered proteins were identified on the corresponding silver-stained 2D gel with testicular proteins (a,d), immunoblotting with control serum (b,e), and immunoblotting with representative EAO serum (c,f). The numbered spots indicated proteins that were identified by MS, Tubb2c (spot no. 2), Atp6v1a (spot no. 10), Pdhb (spot no. 15), Hsc70t (spot no. 16), Fbp1 (spot no. 21), Lrrc34 (spot no. 24), Dnpep (spot no. 27), Gapdhs (spot no. 32), Pdha2 (spot no. 34), Dazap1 (spot no. 35), unnamed protein product (spot no. 36) were detected as protein relevant to EAO among the spots. The apparent molecular masses and pIs of the autoreactive proteins were determined by matching them with standard proteins with known pIs and molecular masses.