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. 2016 Oct 14;7:13189. doi: 10.1038/ncomms13189

Figure 4. Activating CaMKII pathway stimulated mPTP opening and mitochondrial stress in adult cardiomyocytes.

Figure 4

(a) Representative images of Western blot using the anti-HA antibody confirmed the expression of HA-tagged wild-type CaMKII (CaMKII WT) and a constitutively active CaMKII (CaMKII CA). (b) Overexpression of CaMKII WT or CaMKII CA significantly increased flash frequency in adult cardiomyocytes. N=26, 28 and 26 cells from four rats in the groups of control, CaMKII WT and CaMKII CA, respectively. *P<0.05 versus control group. (c) Inhibition of CaMKII activity by applying KN93 (0.5 μM) attenuated the increased flash frequency by CaMKII WT overexpression. N=7, 9 and 9 cells from three rats in the groups of Control, CaMKII WT and WT+KN93, respectively. *P<0.05 versus control group, #P<0.05 versus CaMKII WT group. (d) CsA (1 μM) inhibited the increased mPTP openings by CaMKII WT overexpression. N=14, 27 and 22 cells from three rats in the groups of control, CaMKII WT and WT+KN93, respectively. *P<0.05 versus Control group, #P<0.05 versus CaMKII WT group. (e) CaMKII WT overexpression promoted laser-induced permanent loss of Δψm in adult cardiomyocytes and showed additive effect with ISO treatment (100 nM, 12 h). N=536, 366, 305 and 242 mitochondria from 21 to 48 cells and four rats in the groups of control, ISO, CaMKII WT and WT+ISO, respectively. *P<0.05 versus Vehicle group, #P<0.05 versus CaMKII WT group. (f) CaMKII WT overexpression exaggerated cell death after ISO treatment (100 nM, 24 h). N=665, 577, 1,216 and 567 cell from five rats in the groups of Vehicle, CaMKII WT, ISO and WT+ISO, respectively. *P<0.05 versus ISO 24 h group. Data in b–f are mean±s.e.m. The data were analysed using One-way ANOVA followed by Turkey post test in b–f.