Figure 7. CaMKII binds and directly phosphorylates Drp1 at S616.

(a) Co-immunoprecipitation analysis showing the binding of Drp1 with CaMKII in adult cardiomyocytes. Images are representative of four repeats. (b) HA-tagged CaMKII from H9C2 cells were attached to anti-HA magnetic beads and incubated with WT Drp1 or S616A mutation purified from E. coli. (2 μM) in the presence or absence of calmodulin (1 mM) and Ca²⁺ (1 mM) for 24 h. The supernatant was used for Western blot to detect Drp1 S616 phosphorylation. Images are representative of three repeats. (c) The beads were boiled and samples were used for Western blot using anti-HA (for CaMKII) or anti-Drp1 antibodies for determining the in vitro interaction between CaMKII and Drp1. Images are representative of three repeats.