Table 1.

List of extraction and purification kits tested in this study^a.

Use	Target	Kit name	Abbreviation	Company
Extraction	DNA	PowerLyzer PowerSoil DNA Isolation Kit	PL	MO BIO Laboratories
Extraction	DNA	FastDNA SPIN Kit for Soil	FDS	MP Biomedicals
Extraction	DNA	ZR Soil Microbe DNA MiniPrep	SM	Zymo Research
Extraction	DNA/RNA	MasterPure RNA Purification Kitb	MP	Epicentre Biotechnologies
Extraction	DNA/RNA	PowerMicrobiome RNA Isolation Kit	PM	MO BIO Laboratories
Extraction	RNA	RNA PowerSoil Total RNA Isolation Kit	PS	MO BIO Laboratories
Extraction	DNA	RNA PowerSoil DNA Elution Accessory Kit (used in conjunction with the above RNA kit)	AK	MO BIO Laboratories
Purification	DNA	E.Z.N.A. Cycle Pure Kit	CP	Omega Bio-Tek
Purification	DNA	MinElute Reaction Cleanup Kit	MRC	QIAGEN
Purification	DNA	Genomic DNA Clean & Concentrator	gDCC	Zymo Research
Purification	RNA	RNeasy Mini Kit	RM	QIAGEN
Purification	RNA	RNA Clean & Concentrator – 5	RCC	Zymo Research
Purification	DNA/RNA	OneStep PCR Inhibitor Removal Kit	OPIR	Zymo Research

^aThe purification kits were tested in combination with the modified method described in this paper.

^bThe lysate was obtained using the phenol-chloroform extraction as detailed previously (Nicolaisen et al., 2008).