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Individual qPCR efficiencies based on LinRegPCR analysis of nucleic acids extracted from soils FH (high pH peat, pH 7.39) and FL (low pH peat, pH 3.65).
| Target | Plasmid standard | FH | FL |
|---|---|---|---|
| 16S rRNA gene | 77.9 ± 3.44% | 81.3 ± 3.18% | 82.0 ± 3.49% |
| nosZ DNA | 84.2 ± 5.05% | 85.4 ± 3.97% | 84.2 ± 3.36% |
| nosZ cDNA | Same as above | 80.7 ± 2.51% | 81.0 ± 2.86% |
