Table 2.

Oligonucleotide primers specific to members of the vAh pathotype (vAh-SerF and vAh-SerR), previously described qPCR vAh primers (2986F and 2986R), and primers used to screen for unique isolates used in this study (ML09-119F, ML09-119R, S14-452F, S14-452R, ZC1F, and ZC1R).

Primer name	Direction	Sequence	Amplicon size (bp)
vAh-SerF	Forward	5′-AG′CATCACCAGCGTTGGCCC-3′	502
vAh-SerR	Reverse	5′-GCCGGGCTGAACTTCCGCAT-3′
2986F	Forward	5′-CTATTACTGCCCCCTCGTTC-3′	167
2986R	Reverse	5′-ATTGAGCGGTATGCTGTCG-3′
ML09-119F	Forward	5′-GTTCCGTTCCATCTGTTCGTGA-3′	246
ML09-119R	Reverse	5′-CAACCATCTTGGTCGCAATC-3′
S14-452F	Forward	5′-CAGAACGTGCTGCAGAGATTGA-3′	350
S14-452R	Reverse	5′-TCCGAGAATTCGATGACGAAGG-3′
ZC1F	Forward	5′-GCAATTCTGCGGTCACTTCTCG-3′	400
ZC1R	Reverse	5′-AGCGTACCGTCTCGTCGATATG-3′