Table 1.
Gene names, primer pair sequences, annealing temperatures, and amplification efficiencies of each target.
| Genea | GenBank accession no. | Primer sequence (5′–3′) | Amplicon (bp) | Efficiency (%) |
|---|---|---|---|---|
| ESR1 | NM_001001443.1 | F-CTCTCTGCCTTTGCTACCTTAC | 114 | 98.87 |
| R-CCCGGGTCACAAATAGCTAAA | ||||
| FSHR | NM_174061.1 | F-CATGCTCATCTTCACCGACTT | 112 | 99.64 |
| R-GACCAGGAGGATCTTTGACTTG | ||||
| LHR | NM_174381.1 | F-GAGTGGCTGGGATTATGACTATG | 127 | 99.87 |
| R-CAGCCAAATCAGGACTCTAAGG | ||||
| BMPR2 | NM_001304285.1 | F-GTACCGGCATGACCACTATATC | 120 | 99.74 |
| R-AGTCTTCTTCGGTCAGTTGTAAG | ||||
| TGFB1 | NM_001166068.1 | F-TGCTTCAGCTCCACAGAAA | 149 | 99.74 |
| R-GTATCCAGGCTCCAGATGTAAG | ||||
| TGFB2 | NM_ 001113252.1 | F-CACGAATGGCTCCACCATAA | 127 | 98.67 |
| R-AGCGTGCTTCTAGTTCTTCAC | ||||
| GDF9 | NM_174681.2 | F-GCATTCCCTCCACCCTAAA | 113 | 99.89 |
| R-GGTGACGGGACAATCTTACA | ||||
| BMP15 | NM_ 001031752.1 | F-GTAGTGAGGTTCGTGAGTTCTG | 111 | 98.85 |
| R-TAGGGAGAGGTTTGGTCTTCT | ||||
| BMP6 | XM_005223892.2 | F-TTCTCAACGACGCCGATATG | 122 | 98.56 |
| R-ACCCTCAGGAATCTGGGATAG | ||||
| BMP7 | NM_ 001206015.1 | F-ATGACAGCTCCAACGTCATC | 123 | 98.99 |
| R-AGAGACCCAGGATCCAGAAA | ||||
| CYP19A1 | NM_174305.1 | F-GTGTCCGAAGTTGTGCCTAT | 101 | 98.95 |
| R-GACCTGGTATTGAGGATGTGTC | ||||
| HSD3B1 | NM_174343.3 | F-ACACCAGCACCATAGAAGTG | 113 | 98.88 |
| R-CTTGCTGTATGGGTATGGAGAG | ||||
| IGF1 | NM_001077828.1 | F-TCCCATCTCCCTGGATTTCT | 105 | 98.86 |
| R-GGGTTGGAAGACTGCTGATT | ||||
| IGFR1 | NM_001244612.1 | F-GTATGGAGGAGCCAAGCTAAA | 123 | 98.98 |
| R-GTCTTGGCCTGAACGTAGAA | ||||
| IGF2 | NM_174087.3 | F-CATTGTGGGAAGGTGTGTC | 113 | 99.02 |
| R-TTACATTCTGTGGGCTGTGG | ||||
| B2M | NM_173893.3 | F-ACCTGAACTGCTATGTGTATGG | 134 | 99.95 |
| R-GTGGGACAGCAGGTAGAAAG | ||||
| GAPDH | NM_001034034.2 | F-GATGCTGGTGCTGAGTATGT | 113 | 99.96 |
| R-GCAGAAGGTGCAGAGATGAT |
aGene symbol: ESR1, estrogen receptor α; FSHR, follicle-stimulating hormone receptor; LHR, luteinizing hormone receptor; BMPR2, bone morphogenetic protein receptor, type 2; TGFβ1, transforming growth factor beta 1; TGFβ2, transforming growth factor beta 2; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; BMP6, bone morphogenetic protein 6; BMP7, bone morphogenetic protein 7; CYP19A1, cytochrome P450 aromatase; HSD3B1, 3-β-hydroxysteroid dehydrogenase; IGF1, insulin-like growth factor 1; IGFR1, type 1 insulin-like growth factor receptor; IGF2, insulin-like growth factor 2; B2M, beta-2-macroglobulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.