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. 2016 Oct 18;6:35617. doi: 10.1038/srep35617

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Copyright © 2016, The Author(s)

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Multiplex PCR targeting genes coding for resistance to chloramphenicol/florfenicol (A), sulfonamides (B), and tetracyclines (C,D). Electrophoresis gels with amplicons generated using DNA isolated from positive controls (A449 + M15879-11 in (A,C), M15879-11 in B, 2009-144K3 + M16474-11 in D) and from a negative control (01-B526). A specific control for A. salmonicida subsp. salmonicida using a primer pair that amplified an element of the prophage 1 was included in each multiplex PCR reaction. The amplicon of this product can be seen at the bottom of each gel (control). (A) The target cat (448 bp) and floR genes (632 bp) and (B) the sul2 (449 bp) and sul1 (550 bp) genes were detected when present. (C) Amplicons for tet(E) (351 bp) and tetA (526 pb). (D) Amplicons for tet(H) (326 bp), tet(G) (460 bp), and tet(C) (629 bp). Water was used as a negative control.