Figure 2. Identification of KRT23 as a Marker of Ductular Cells in AH.
(a) Representative micrographs of KRT23 protein expression in paraffin sections of liver biopsies from normal livers and from patients with AH, stained with anti-KRT23 antibody (x100 magnification). (b) Immunofluorescence staining shows co-localization of KRT23 and LPCs markers (KRT7 and EPCAM) in paraffin sections of liver biopsies from patients with AH (x100 magnification). (c) Krt23 hepatic gene expression measured in a mouse model of progenitor cell expansion (DDC diet for 4 weeks, n = 6 compared to uninjured mouse liver (control, n = 6). (d) Representative images of liver KRT23 staining in mice treated for 4 weeks with a DDC diet and in uninjured mice (x200 magnification). (e) Krt23 and LPCs markers (Epcam, Krt7, Krt19) gene expression was significantly enriched in LCP cells (n = 3) vs. a non-LPC population (n = 3).