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. 2016 Feb 13;14(9):1826–1837. doi: 10.1111/pbi.12540

Figure 9.

Figure 9

Effect of tunicamycin (TM) on the phenotype of transgenic sugarcane. Plants with 4 weeks of age were incubated in different treatments: T1 (control without TM), T2 (0.5 mg/L TM) and T3 (1.0 mg/L TM) for 10 days. (a) Effect of TM on the sugarcane root elongation of WT plants and transgenic plants SCBI‐3 and ScBI‐4. The vertical length of the root system (mm) was determined at 1st, 4th, 7th and 10th days after the start of the experiment. Error bars indicate standard deviation of 10 biological samples. (b) Phenotype of the sugarcane root system of WT plants and transgenic plants (ScBI‐3 and ScBI‐4) incubated in different dosage of the antibiotic, 10th day after treatments. In the black window: swelling of root tips at T2. (c) Chlorophyll content in WT and transgenic plants (ScBI) growing in liquid MS medium with tunicamycin, after 12 days of incubation. Error bars indicate standard deviation of 10 biological samples. (d) Phenotype of sugarcane growing in liquid MS medium with tunicamycin. Arrows indicates WT plantlets with leaves and roots browning after 12 days of incubation with 1.0 mg/L of TM (T3 condition).