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. 2016 Oct 18;6:35468. doi: 10.1038/srep35468

Figure 8. APG and TRAIL combined therapy induces necrosis of tumor and promotes tumor cell apoptosis.

Figure 8

(a) Determination of tumor necrosis and apoptosis after combined treatment with APG and TRAIL. Tumor necrosis areas are shown by H&E staining and observed under light microscope (×100). The viable tumor cells are indicated by a blue arrow. TUNEL assay was used to detect apoptotic cells (original magnification, ×200). Positive cells for TUNEL staining are indicated by a green arrow. (b) Quota of tumor necrosis and apoptosis. Tumor necrosis was determined by software Image J. Two sections/mouse and three mice were prepared (mean ± SD, *P < 0.05, **p < 0.01). The ratio of apoptotic cells to total cells: TUNEL positive cells were counted from three fields of the highest density of positive-stained cells in each section to determine the percentage of apoptotic cells (mean ± SD, *p < 0.05, **p < 0.01).