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. 2016 Oct 18;6:35655. doi: 10.1038/srep35655

Figure 6. Osterix represses rosiglitazone-induced adipogenesis.

Figure 6

(A) 3T3-L1 cells were transfected with an empty vector or Myc-Osterix expression plasmid. Cells were then cultured with or without 1 μM rosiglitazone (RSG) for 8 days. The mRNA expression of Pparγ, ap2, Adiponectin, and Glut4 was determined by real-time PCR and normalized to that of Gapdh. *P < 0.05 compared to the control. #P < 0.05 compared to samples treated only with rosiglitazone (RSG); ANOVA–Bonferroni post hoc test, n = 3. (B,C) 3T3-L1 cells were co-transfected with indicated combinations of HA-PPARγ (0.2 μg) and Myc-Osterix (0.2 μg) along with PPRE-Luc or aP2-Luc reporters. (D,E) 3T3-L1 cells were co-transfected with indicated combinations of HA-PPARγ (0.2 μg) and shRNA to Osterix (0.2 μg) along with PPRE-Luc or aP2-Luc reporter. After transfection (24 h), cells were treated with or without 1 μM rosiglitazone (RSG) and cultured for additional 12 h. The expression of the reporter was measured using a luciferase assay. *P < 0.05, **P < 0.01, and ***P < 0.001; Student’s t-test, n = 3.