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. 2016 Oct 18;6:35655. doi: 10.1038/srep35655

Table 1. Sequences of primers used for PCR.

  Gene Primer sequence (5′→3′)
PCR Osterix (F) 5′-GGC ACA AAG AAG CCG TAC TC-3′
(R) 5′-GCC TTG TAC CAG GAG CCA TA-3′
C/EBPβ (F) 5′-AGC CCC TAC CTG GAG CCG CT-3′
(R) 5′-GCG CAG GGC GAA CGG GAA AC-3′
C/EBPα (F) 5′-TGC TGG AGT TGA CCA GTG AC-3′
(R) 5′-AAA CCA TCC TCT GGG TCT CC-3′
PPARγ (F) 5′-ATC AGC TCT GTG GAC CTC TC-3′
(R) 5′-ACC TGA TGG CAT TGT GAG AC-3′
GAPDH (F) 5′-ACC ACA GTC CAT GCC ATC AC-3′
(R) 5′-TCC ACC ACC CTG TTG CTG TA-3′
qPCR Osterix (F) 5′-AGC GAC CAC TTG AGC AAA CAT-3′
(R) 5′-GCG GCT GAT TGG CTT CTT CT-3′
PPARγ (F) 5′-GCC CTT TGG TGA CTT TAT GGA-3′
(R) 5′-GCA GCA GGT TGT CTT GGA TG-3′
aP2 (F) 5′-GAA CCT GGA AGC TTG TC-3′
(R) 5′-CGT GAC TTC CAC AAG AGT-3′
Adiponectin (F) 5′-CCC AGG ACA TCC TGG CCA CAA-3′
(R) 5′-CCT TCA GCT CCT GTC ATT CCA-3′
GluT4 (F) 5′-GTA ACT TCA TTG TCG GCA TGG-3′
(R) 5′-AGC TGA GAT CTG GTC AAA CG-3′
GluT1 (F) 5′-TCA ACA CGG CCT TCA CTG-3′
(R) 5′-CAC GAT GCT CAG ATA GGA CAT C-3′
GAPDH (F) 5′-AGG TCG GTG TGA ACG GAT TTG-3′
(R) 5′-GGG GTC GTT GAT GGC AAC A-3′