PCR |
Osterix |
(F) 5′-GGC ACA AAG AAG CCG TAC TC-3′ |
(R) 5′-GCC TTG TAC CAG GAG CCA TA-3′ |
C/EBPβ |
(F) 5′-AGC CCC TAC CTG GAG CCG CT-3′ |
(R) 5′-GCG CAG GGC GAA CGG GAA AC-3′ |
C/EBPα |
(F) 5′-TGC TGG AGT TGA CCA GTG AC-3′ |
(R) 5′-AAA CCA TCC TCT GGG TCT CC-3′ |
PPARγ |
(F) 5′-ATC AGC TCT GTG GAC CTC TC-3′ |
(R) 5′-ACC TGA TGG CAT TGT GAG AC-3′ |
GAPDH |
(F) 5′-ACC ACA GTC CAT GCC ATC AC-3′ |
(R) 5′-TCC ACC ACC CTG TTG CTG TA-3′ |
qPCR |
Osterix |
(F) 5′-AGC GAC CAC TTG AGC AAA CAT-3′ |
(R) 5′-GCG GCT GAT TGG CTT CTT CT-3′ |
PPARγ |
(F) 5′-GCC CTT TGG TGA CTT TAT GGA-3′ |
(R) 5′-GCA GCA GGT TGT CTT GGA TG-3′ |
aP2 |
(F) 5′-GAA CCT GGA AGC TTG TC-3′ |
(R) 5′-CGT GAC TTC CAC AAG AGT-3′ |
Adiponectin |
(F) 5′-CCC AGG ACA TCC TGG CCA CAA-3′ |
(R) 5′-CCT TCA GCT CCT GTC ATT CCA-3′ |
GluT4 |
(F) 5′-GTA ACT TCA TTG TCG GCA TGG-3′ |
(R) 5′-AGC TGA GAT CTG GTC AAA CG-3′ |
GluT1 |
(F) 5′-TCA ACA CGG CCT TCA CTG-3′ |
(R) 5′-CAC GAT GCT CAG ATA GGA CAT C-3′ |
GAPDH |
(F) 5′-AGG TCG GTG TGA ACG GAT TTG-3′ |
(R) 5′-GGG GTC GTT GAT GGC AAC A-3′ |