Figure 1. KaiC_DT predominantly binds to KaiB.

KaiC_WT, KaiC_DE, KaiC_DT, KaiC_AA, and KaiC_SE were incubated with KaiB at 30 °C for 12 h and were subjected to BN-PAGE. The upper and lower bands with respect to the 480-kDa position corresponded to KaiB–KaiC complex and ground-state (gs)-KaiC, respectively. The 66-kDa protein bands corresponded to the competent state (cs)-KaiC. The gs-KaiC and cs-KaiC were donated in the previous work²¹. The full-length gels of BN-PAGE are presented in Supplemental Fig. S1.