FIG 3.

VitD deficiency does not cause changes in response to acute infection with viable P. aeruginosa PAO1. Mice were infected intranasally with viable P. aeruginosa PAO1 (1.3 × 10⁶ to 4.95 × 10⁶ CFU/mouse; n = 10, two replications). (A) The numbers of viable bacteria in BALF and lungs were counted after 6 h or 24 h. No differences between the two groups were found. (B to D) The two groups exhibited similar numbers of total cells, neutrophils, and macrophages in BALF. (E to G) The levels of cytokines were measured by ELISA, and no differences were observed in the concentrations of KC in BALF or in lung homogenates (E and F) and of IL-1β in lung homogenates (G). Statistical analysis was performed by ANOVA and t test between control and malnutrition groups with no significant differences.