FIG 6.
VitD deficiency does not result in alterations of the response after subacute infection with S. pneumoniae D39. Mice were analyzed 24 h after intranasal stimulation with two different doses of viable S. pneumoniae D39 (8.8 × 105 or 2.94 × 106 CFU/mouse; n = 10, two replications). (A) The numbers of viable bacteria in BALF and lungs were counted. No differences between the VitD-deficient and -sufficient animals were found. (B to D) The two groups exhibited similar numbers of total cells, neutrophils, and macrophages in BALF. (E to G) The levels of cytokines were measured by ELISA, and no differences were observed in the concentrations of KC in BALF or in lung homogenates (E and F) and of IL-1β in lung homogenates (G). Statistical analysis was performed by ANOVA and t test between control and malnutrition groups with no significant differences.