FIG 2.

HPLC chromatograms of serotype 19A subtypes and 19F strain B201.73 grown in CDM and PIM. Results from HPLC composition analysis of hydrolyzed polysaccharide capsule of clinical isolates of serotype 19A subtypes grown in chemically defined medium with 55 mM glucose (A) compared to ATCC purified pneumococcal serotype 19A polysaccharide (B), and the pneumococcal inoculation medium PIM (C) are shown. The y axis shows fluorescence (fluorescence units), and chromatograms were stacked to facilitate comparison. Peaks of mannosamine (Man-N), rhamnose (Rha), and glucose (Glc) are labeled.