FIG 1.

TRP32 localizes to the nucleus of E. chaffeensis-infected THP-1 cells. (A) E. chaffeensis-infected and uninfected THP-1 cells were fixed and probed with rabbit anti-TRP32 (green), anti-DSB (red, morula), and DAPI (blue, DNA) and then visualized using confocal microscopy. Early (24 hpi) TRP32 is associated primarily with the morulae. At 48 hpi, TRP32 localized with morulae and in the perinuclear region. At 72 hpi, TRP32 localized with the morulae but was also observed at the perinuclear region and in the nucleus of the host cell. TRP32 was not observed in uninfected cells. Bar, 10 μm. (B) Orthogonal projections of optical slices from a z-stack of an E. chaffeensis-infected THP-1 cell at 72 hpi showed both diffuse and punctate TRP32 within the host nucleus. Top panels show a y-z projection with left panels showing an x-z projection. The positions of the x and y axes within the projections denote the z depth of the slice shown in the center.