FIG 10.

E. chaffeensis TRP32 has a direct effect on target gene expression. (A) TRP32 targets are differentially regulated during infection. The most highly enriched genes from the ChIP-seq were assayed by qPCR at 24, 48, and 72 hpi. Data are presented as fold changes from noninfected. Colors represent the fold changes from −10 to +10. Data are representative of two (24 and 48 hpi) or three (72 hpi) experiments. (B) TRP32 modulates gene expression in a luciferase reporter assay. Several genes were chosen, and the promoter regions, including the TRP32 binding site, were cloned into a luciferase reporter. Promoter constructs were transfected into HeLa cells along with various concentrations of a TRP32-expressing or empty GFP vector. Luciferase expression was measured after 24 h. Luciferase expression is reported as fold change from control. Data are representative of 3 to 5 experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.