FIG 3.

Cysts are undetectable before day 7 postinfection in BALB/cJ adults infected with P. murina trophic forms. Adult mice were infected i.t. with 5 × 10⁶ trophic forms or mixed P. murina organisms. Trophic (A and C) and cystic (B and D) lung burdens from two separate experiments were determined by enumeration of organisms on Diff-Quik-stained slides under a microscope. Data are means ± standard deviations for 5 mice per group. The limit of detection was log₁₀ 3.42 nuclei per adult right lung lobe. For comparison of mean fungal burdens between the two groups at individual time points, t tests were used. *, P ≤ 0.05.