TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence (5′–3′)a	Product size; positions or relevant characteristicb
ermE1-AscI	TTGGCGCGCCTGGCGGAAACGTAAAAGAAG	998 bp; amplicon containing the Ermr gene from pVA838
ermE2-XhoI	TTCTCGAGGGCTCCTTGGAAGCTGTCAGT
gbpCP1	CCCTCAACACACTCTGCTAA	473 bp; 323 bp upstream to 150 bp downstream of the gbpC ORF
gbpCP2-AscI	TTGGCGCGCCCGGTTCTGATGCTTGTGTAT
gbpCP3-XhoI	TTCTCGAGGGAGAAATGCGTGTTAGAGA	387 bp; 1,605 bp upstream to 240 bp downstream of the encoding region of gbpC
gbpCP4	CTTACCCATCACAAAAACCA
C1-SacI	GGGAGCTCCCCTCAACACACTCTGCTAA	2,139 bp; amplicon containing the encoding region of gbpC for mutant complementation
C2-SphI	GGGCATGCAACAAGAACTGCTGCTCAAG

^aUnderlined sequences indicate restriction enzyme linkers.

^bORF, open reading frame.