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. 2016 Oct 17;84(11):3232–3242. doi: 10.1128/IAI.00749-16

FIG 1.

FIG 1

Characterization of genital tract immune cellular infiltrate during primary C. muridarum infection. Whole genital tracts were collected from CD4-depleted and nondepleted naive C57BL/6 mice (A) and at 7 days (B) and 21 days (C) following intravaginal infection. Viable cells were isolated for flow cytometry and stained with antibodies representing the following immune populations (as detailed in Materials and Methods): CD4+ T cells (CD4+), CD8+ T cells (CD8+), dendritic cells (CD11c+), B cells (CD45R+), NK cells (NK1.1+), macrophages (CD11b+ F4/80+), and neutrophils (CD11b+ Ly6G+). Data represent the mean number of positive-staining cells per genital tract ± standard deviations (SD) from 9 mice/group.