FIG 6.

Effect of CD4⁺ T cell depletion on the activation state of the murine genital tract during primary C. muridarum infection. RNA was harvested from whole genital tracts from CD4-depleted or nondepleted C57BL/6 mice (n = 4) at the noted time points during primary infection for analysis by quantitative reverse transcriptase PCR. Expression of activation-related mRNAs was determined. (A) Inducible nitric oxide synthase (NOS2) and arginase 1 (Arg1) total transcript numbers per genital tract in naive, CD4-depleted, and nondepleted mice were calculated. (B) mRNA levels of IL-10, Ym1, Fizz1, IL-6, and TNF-α in CD4-depleted and nondepleted C57BL/6 mice relative to naive controls were determined. Data were normalized to the HPRT1 housekeeping gene. Numbers represent means ± SD (n = 4).