FIG 5.

(A) Survival plot of mice given intraperitoneal injections containing 10⁵ WT (●) or ΔwcaM ΔcsgA ΔyihO ΔbcsE mutant (■) cells. All mice were sacrificed at day 8 postinfection. n = 8 mice/group. (B) CFU enumeration in gallbladder or bile or on gallstones from mice given intraperitoneal injections containing 10⁴ WT (●) or ΔwcaM ΔcsgA ΔyihO ΔbcsE mutant (■) cells at 8 days postinfection in the presence of gallstones. The limit of detection was 100 CFU/ml. An unpaired t test with Welch's correction was used to determine significant differences between the mutant strain and WT (**, P < 0.01). Bars indicate means. (C) CFU enumeration in gallbladder or bile or on gallstones from mice given intraperitoneal injections containing 10⁴ WT (●) or ΔwcaM ΔcsgA (◆), ΔwcaM ΔcsgA ΔyihO (▲), or ΔwcaM ΔcsgA ΔyihO ΔbcsE (■) mutant cells at 8 days postinfection in the presence of gallstones. The limit of detection was 100 CFU/ml. One-way ANOVA with Dunnett's multiple comparison test was used to determine significant differences between mutant strains and the WT (*, P < 0.05). Bars indicate means. (D) Bacterial CFU enumeration of Salmonella at 8 days postinfection in the presence of gallstones. Mice were challenged with intraperitoneal injections containing a 10⁴-CFU mixture of WT cells marked with streptomycin resistance and ΔwcaM ΔcsgA ΔyihO ΔbcsE mutant cells marked with chloramphenicol. The limit of detection was 100 CFU/ml. The dotted line represents a completely neutral competitive index. Wilcoxon's signed rank test was used to determine significant differences between mutant strains and the WT (*, P < 0.05; **, P < 0.01). Bars indicate means. Positive values represent the mutant outcompeting the WT.