FIG. 2.

CCL1 production by CCL1⁺CD163⁺CD14⁺ cells treated with synthesized ODNs. IL-1β/IgG-CCL1⁺CD163⁺CD14⁺ cells (3 × 10⁵ cells/mL) were cultured with the ODNs for 2 days. Cells were harvested and recultured with fresh medium for 24 h. Culture fluids were harvested and assayed for CCL1 by ELISA. (A) CCL1 production in IL-1β/IgG-CCL1⁺CD163⁺CD14⁺ cell cultures supplemented with 4 or 20 μg/mL of the ODNs. ○, synthesized ODN; ●, AS1. (B) Summary in the results of (A) experiments. Data are shown as mean ± SD. *P < 0.05 compared with AS1. (C) CCL1 production by IL-1β/IgG-CCL1⁺CD163⁺CD14⁺ cells treated with 20 μg/mL of HCA-11 or scrambled ODN. ELISA, enzyme-linked immunosorbent assay; SD, standard deviation.