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. 2016 Oct 17;10:13. doi: 10.1186/s13036-016-0035-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Comparison of B. subtilis mutants with the wild type strain for alkaline amylase production and strain growth. a alkaline amylase production. The inner is SDS-PAGE analysis. Arrow: alkaline amylase, 1: Wild type B. subtilis 168, 2: B. subtilis 168 mut-16#, M: standard protein marker. b specific production rate. c DCW. d specific growth rate