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. 2016 Oct 18;4:65. doi: 10.1186/s40425-016-0163-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — 5-FU treatment promotes B7-H1 protein expression in whole cell lysates from HCT 116 WT ^+/+ and HCT 116 p53 ^−/− colon cancer cell lines. a Western blots from seven independent experiments measuring B7-H1 and actin expression in HCT 116 p53^+/+ and HCT 116 p53^−/− cells. The “X” in experiment # 2 indicates a skipped lane when loading the samples. Unt = untreated control, 0.5, 5, 50 and 500 = 5FU doses in uM, IFN = 10 ng/ul of interferon gamma. b Quantification of B7-H1 expression using ImageJ. Data is represented as mean B7-H1 band intensity/mean actin band intensity +/− SEM. *** p < 0.001 relative to the untreated control